Highly sensitive detection of nucleocapsid protein from SARS-CoV-2 using a near-infrared surface plasmon resonance sensing system

Author:

Kajisa Taira12ORCID,Yano Taka-aki,Koresawa Hidenori,Otsuka Kunihiro2,Sakane Ayuko2,Sasaki Takuya2,Yasutomo Koji2,Yasui TakeshiORCID

Affiliation:

1. Toyo University

2. Tokushima University

Abstract

We report a near-infrared surface plasmon resonance (SPR) system to achieve highly sensitive, unlabeled detection of the SARS-CoV-2 nucleocapsid protein antigen. Use of the near-infrared light in SPR makes the SPR dip of the angular spectrum sharp and causes a large change of the reflected light intensity at a fixed incident angle. The present SPR system achieves the resolution of 10−5 refractive index unit in the refractive index measurement of glycerol solution samples. Additionally, we measured the nucleocapsid protein antigen of SARS-CoV-2 down to a molar concentration of 1 fM by immobilizing its corresponding antibody on the SPR sensor surface. This demonstration indicates a high potential of the present system for highly sensitive biosensing in medical diagnostics.

Funder

Japan Agency for Medical Research and Development

Japan Society for the Promotion of Science

Publisher

Optica Publishing Group

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