Light-field micro-endoscopy using a fiber bundle: a snapshot 3D epi-fluorescence endoscope

Author:

Zhou YouORCID,Xiong Bo1ORCID,Song Weizhi,Zhang Xu2,Zheng Guoan3,Dai Qionghai1ORCID,Cao Xun

Affiliation:

1. Tsinghua University

2. Beijing Institute of Collaborative Innovation

3. University of Connecticut

Abstract

Micro-endoscopes are widely used for detecting and visualizing hard-to-reach areas of the human body and for in vivo observation of animals. A micro-endoscope that can realize 3D imaging at the camera framerate could benefit various clinical and biological applications. In this work, we report the development of a compact light-field micro-endoscope (LFME) that can obtain snapshot 3D fluorescence imaging, by jointly using a single-mode fiber bundle and a small-size light-field configuration. To demonstrate the real imaging performance of our method, we put a resolution chart in different z positions and capture the z-stack images successively for reconstruction, achieving 333-μm-diameter field of view, 24 μm optimal depth of field, and up to 3.91 μm spatial resolution near the focal plane. We also test our method on a human skin tissue section and HeLa cells. Our LFME prototype provides epi-fluorescence imaging ability with a relatively small (2-mm-diameter) imaging probe, making it suitable for in vivo detection of brain activity and gastrointestinal diseases of animals.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials

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