Longitudinal monitoring of pancreatic islet damage in streptozotocin-treated mice with optical coherence microscopy

Author:

Park Won Yeong,Kim Jaeyoon1,Le Hoan,Kim Bumju,Berggren Per-Olof12,Kim Ki HeanORCID

Affiliation:

1. Pohang University of Science and Technology

2. Karolinska Institutet

Abstract

Pancreatic islets regulate glucose homeostasis in the body, and their dysfunction is closely related to diabetes. Islet transplantation into the anterior chamber of the eye (ACE) was recently developed for both in vivo islet study and diabetes treatment. Optical coherence microscopy (OCM) was previously used to monitor ACE transplanted islets in non-obese diabetic (NOD) mice for detecting autoimmune attack. In this study, OCM was applied to streptozotocin (STZ)-induced diabetic mouse models for the early detection of islet damage. A custom extended-focus OCM (xfOCM) was used to image islet grafts in the ACE longitudinally during STZ-induced beta cell destruction together with conventional bright-field (BF) imaging and invasive glucose level measurement. xfOCM detected local structural changes and vascular degradation during the islet damage which was confirmed by confocal imaging of extracted islet grafts. xfOCM detection of islet damage was more sensitive than BF imaging and glucose measurement. Longitudinal xfOCM images of islet grafts were quantitatively analyzed. All these results showed that xfOCM could be used as a non-invasive and sensitive monitoring method for the early detection of deficient islet grafts in the ACE with potential applications to human subjects.

Funder

National Research Foundation of Korea

Korea Medical Device Development Fund

Vetenskapsrådet

Family Erling-Persson Foundation

Stichting af Jochnick Foundation

Swedish Diabetes Association

Berth von Kantzow’s Foundation

European Research Council

Stiftelsen för Strategisk Forskning

Knut och Alice Wallenbergs Stiftelse

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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