Abstract
The germplasm of Passiflora L. is conserved through seed banks and field collections. Dormant seeds, seeds with low viability, and high-cost field collections make it difficult to maintain germplasm banks, and in vitro conservation can be a complementary alternative. The aim of this study was to investigate the survival of nodal segments of Passiflora edulis Sims ‘UENF Rio Dourado’ over 180 days of slow growth in vitro by reducing the mineral salt and sucrose concentrations and changing the incubation conditions (temperature and light intensity). The experiment was conducted in a completely randomized design with a 2 × 3 × 3 factorial arrangement consisting of two temperatures (20 ± 2°C and 27 ± 2°C), three concentrations of MSM mineral salts (100% MSM, 50% MSM, and 25% MSM), and three sucrose concentrations (10, 20, and 30 g L-1). Evaluations were performed at 60, 90, 120, 150, and 180 days for survival, number of leaves, and plant color (using a color scale where 1 = dark green, 2 = light green, and 3 = yellow). After 180 days of culture, mineral salt, sucrose concentration, and incubation temperature affected plant survival, regeneration, and acclimatization. During the 180 days, it was possible to slow the growth of nodal segments of passion fruit ‘UENF Rio Dourado’ in culture medium with 25% MSM mineral salts plus 10 g L-1 sucrose, at an average temperature of 20°C without compromising plant survival, regeneration, and acclimatization.
Publisher
Universidade Estadual de Maringa
Subject
Agronomy and Crop Science
Reference34 articles.
1. Aguacía, L. M., Miranda, D., & Carranza, C. (2015). Effect of fruit maturity stage and fermentation period on the germination of passion fruit (Passiflora edulis f. flavicarpa Deg.) and sweet granadilla seeds (Passiflora ligularis Juss.). Agronomía Colombiana, 33(3), 305-314. DOI: https://doi.org/10.15446/agron.colomb.v33n3.52460
2. Bernacci, L. C., Cervi, A. C., Milward-de-Azevedo, M. A., Nunes, T. S., Imig, D. C., & Mezzonato, A. C. (2015). Passifloraceae. In Lista de espécies da flora do Brasil. Rio de Janeiro, RJ: Jardim Botânico do Rio de Janeiro. Retrieved on May 10, 2021 from http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB12506
3. Cerqueira-Silva, C. B. M., Faleiro, F. G., Jesus, O. N., Santos, E. S. L., & Souza, A. P. (2016). The genetic diversity, conservation, and use of passion Fruit (Passiflora spp.). In M. R. Ahuja, & S. M. Jain (Eds.), Genetic diversity and erosion in plants (p. 215–231). New York, NY: Springer International Publishing. DOI: https://doi.org/10.1007/978-3319-25954-3_5
4. Delanoy, M., Van Damme, P., Scheldeman, X., & Beltran, J. (2006). Germination of Passiflora mollissima (Kunth) LH Bailey, Passiflora tricuspis Mast. and Passiflora nov sp. seeds. Scientia Horticulturae, 110(2), 198-203. DOI: http://dx.doi.org/10.1016/j. scienta.2006.07.007
5. Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology, 47(1), 5-16. DOI: https://doi.org/10.1007/s11627-010-9327-2