Affiliation:
1. M.V. Lomonosov Moscow State University
Abstract
The article is devoted to the development of a simple chromatographic method for the simultaneous determination of the well-known antiseptics – chlorhexidine and triclosan – in new generation of antiseptic liquids. The
dependences of the retention times of chlorhexidine and triclosan on the acetonitrile
concentration in the mobile phase (from 30 to 60%) and pH (in the range of 3–8
units) for sorbents Luna C18, Nucleosil CN, Diaspher C4 were studied. A perfect
separation of chlorhexidine, p-chloraniline (the most toxic product of chlorhexidine
decomposition), dibutylphthalate (as a component of cosmetic alcohol), triclosan and
ionol (butylhydroxytoluene, BHT, E321 – antioxidant agent) during 20 minutes in the
gradient elution mode on the column with a domestic sorbent Diaspher C4 has been
demonstrated.