Affiliation:
1. Lomonosov Moscow State University
Abstract
For human and chicken lysozyme , the relationship between changes in the parameters of enzyme adsorption on living Escherichia coli bacterial cells and the value of its effective bacteriolytic activity in the presence of glycine and charged amino acids was studied . It has been shown for both human and chicken lysozyme that free amino acids added to a concentration of 1.5 mM for glycine or 5.0 mM for glutamate, aspartate, histidine, arginine, and lysine reduce the desorption constant of the enzyme on bacterial cells to 1.4-2.0 times. At the same time, an increase in the bacteriolytic activity of lysozyme is also observed in 1.5-1.9 times. Thus, the enhancement of antibacterial activity in the presence of glycine and charged amino acids can be explained by an improvement in the productive sorption of the enzyme on the substrate, bacterial cells.
Funder
Lomonosov Moscow State University