Growth of mesenchymal stem cells on oriented microstructured films and electrospun scaffolds

Author:

Demianova I.V.1ORCID,Akoulina E.A.2ORCID,Zharkova I.I.3ORCID,Voinova V.V.3ORCID,Chesnokova D.V.3ORCID,Hossain A.M.3,Makhina T.K.4ORCID,Bonartseva G.A.4ORCID,Kulikouskaya V.I.5ORCID,Nikolaichuk V.V.5,Mukhortova Yu.R.6,Pryadko A.S.6ORCID,Surmeneva M.A.7ORCID,Surmenev R.A.7ORCID,Shaitan K.V.3ORCID,Bonartsev A.P.3ORCID

Affiliation:

1. Shenzhen MSU-BIT University

2. Shenzhen MSU-BIT University; Lomonosov Moscow State University

3. Lomonosov Moscow State University

4. Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Science

5. Institute of Chemistry of New Materials, National Academy of Sciences of Belarus

6. Physical Materials Science and Composite Materials Research Centre

7. Physical Materials Science and Composite Materials Research Centre; Piezo- and Magnetoelectric Materials Research Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University

Abstract

The study involved the fabrication of films with different roughness and scaffolds made of poly(3-hydroxybutyrate) using various methods. Chaotic and oriented scaffolds with varying fiber thickness were obtained through the electrospinning method, depending on the polymer concentration and electrospinning parameters. Films with different surface roughness were obtained using spin coating and self-assembly methods. It was demonstrated that the varying microstructure of the surface does not affect the growth of mesenchymal stem cells over the course of a week; however, it does influence the morphology of the adhered cells.

Funder

Russian Science Foundation

Lomonosov Moscow State University

Publisher

Moscow University Press

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