Affiliation:
1. Lomonosov Moscow State University
2. Pskov State University
Abstract
High-resolution chlorophyll fluorescence light induction curves (OJIP transients) are widely
used to assess the primary photosynthetic responses of phototrophic microorganisms.
Chlorophyll fluorescence measuring methods coupled with microscopy techniques provide a promising opportunity to measure OJIP transients on individual algal cells, allowing scientists
to investigate stress adaptation mechanisms related to reorganization of microalgae population
or phytoplankton community. In this work, we first characterized the OJIP transients measured
on individual algae cells using the original microfluorimeter and compared them with OJIP
transients recorded in microalgae suspensions. Based on the results of the study, we proposed
a method for analyzing OJIP curves of individual microalgae cells as well as ways to further
improve microfluorimeters.
Funder
Russian Science Foundation
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