Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6)-Reference-Cited by-同舟云学术

Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6)

Published:2022-09-14 Issue:1 Volume:2 Page:
ISSN:2737-7466
Container-title:Zoonoses
language:en
Short-container-title:Zoonoses

Author:

Shi Bochang¹²,Li Xinyu³,Li Bo²,Zheng Naxin²,Li Min⁴⁵,Liu Yuan²⁶,Li Chunhe⁴⁷,Yan Fang⁶,He Wei³,Zhao Liyan⁸,Li Huabin⁶,Tian Chongyu⁶,Zheng Yuanqiang¹,Zhao Zhongpeng³

Affiliation:

1. Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical University, Huhehaote Inner Mongolia 010058, China

2. The Fifth Medical Center of PLA General Hospital, Beijing 100039, China

3. Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China

4. Inner Mongolia Huaxi Biotechnology Co, Ltd., Inner Mongolia 010010, China

5. Ximu Bio-technology Co., LTD, Beijing 100010, China

6. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu Shanxi 030800, China

7. State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Huhehaote Inner Mongolia 010070, China

8. People’s Liberation Army Navy’s No.971 Hospital, Qingdao Shandong 266071, China

Abstract

Objective: Brucellosis is a serious zoonotic infection worldwide. To date, vaccination is the most effective measure against brucellosis. This study was aimed at obtaining a vaccine strain that has high protective efficacy and low toxicity, and allows vaccination to be differentiated from infection. Methods: Using homologous recombination, we constructed a double gene-deletion Brucella strain MB6 Δbp26ΔwboA (RM6) and evaluated its characteristics, safety and efficacy. Results: The RM6 strain had good proliferative ability and stable biological characteristics in vivo and in vitro. Moreover, it had a favorable safety profile and elicited specific immune responses in mice and sheep. Conclusion: The RM6 strain may have substantial practical application value.

Publisher

Compuscript, Ltd.

Link

https://scienceopen.com/document_file/8fe3d2af-4a9c-40d9-afb1-4929997af502/ScienceOpen/Zoonoses20220031.pdf

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