The Relationship between IL-1β [-31 T/C] Gene Polymorphism and Symptomatic Lumbar Disc Herniation

Abstract

In this study, the relationship between IL-1β [-31 C / T] gene polymorphisms in the herniated lumbar disc is offered to be investigated. IL-1β [-31 C / T] gene was amplified by the PCR [Polymerase Chain Reaction] method. Polymorphic site cut with the restriction enzyme Alu I and genotyping were performed. Fifteen subjects (15%) were homozygous for the C/C allele at -31 position of IL-1β gene. Of the remaining 85 subjects, 64 (64%) were heterozygous for C/T and 21 (21%) were homozygous for T/T. In LDH group, fifteen subjects (30%) were homozygous for the T/T allele at -31 position of IL-1β gene. 35 subjects were heterozygous for C/T (70%). There were significant differences between control and LDH group. In LDH group, C/T and T/T genotype were higher than the control group, however in control group C / C genotype was higher. C/C genotype was not determined in LDH group. In this study, IL-1β -31T allele is shown to be associated with the Lumbar herniated disc.