The Effects of Acute Toxicity of Dieldrin on HeLa Cell Line: An In Vitro Assessment

Abstract

Aim: Among environmental pollutants, there is a great concern about organochlorine pesticides (OCPs) due to their environmental persistence, accumulation in the food chain, detection in breast milk, and their ability to accumulate in adipose tissues. Due to the toxicity of OCPs and its relationship with human health, this study aimed to investigate the effects of dieldrin pesticides on morphological changes in the HeLa cell line. Materials and Methods: Standard concentrations of dieldrin (0.1-20 ppm) were prepared and cells were cultured in 1640 Roswell Park Memorial Institute (RPMI) medium containing 10% bovine serum albumin and Pen-Strep antibiotic. Subsequently, the morphological effects of dieldrin on HeLa cells in a cell culture medium were investigated. Results: Morphological and cytopathic changes were not observed in HeLa cells treated with concentrations of 0.1, 0.5, and 1 ppm of dieldrin. However, significant changes including cell rounding and cytopathic effects were observed in the cells treated with 5 ppm of dieldrin. Moreover, at concentrations of 15 and 20 ppm of dieldrin, the cells were completely destroyed and could not be examined. Conclusion: The effects of dieldrin on HeLa cell morphology were observed in the form of cell rounding and cytopathic effects. These morphological changes suggest that dieldrin may induce the process of apoptosis in cells. According to the results, the identification of different factors that aggravate the cytotoxic effects of this pesticide needs further research.