A pharmacological perspectives of Musa sapientum peels against lung cancer: An in vitro and in silico study

Abstract

ABSTRACTS Context: The essential role of medicinal plants is studied over 5000 years against the life-threatening diseases such as cancer in developing countries. The more cognizance on molecular mechanism will engender trend to use them efficaciously. Aims: To analyze the pharmacological activity of banana peel against lung cancer. Settings and Design: Banana fruit is a nutritious victuals with proven medicinal properties. The underlying molecular mechanism of phytochemicals present in the banana peel was studied using in vitro and in silico methods to explore an efficacious anticancer drug against lung cancer. Methods and Materials: The petroleum ether extract of Musa sapientum peel is analyzed using gas chromatography–mass spectrometry, in vitro studies using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and lipid peroxidase assay, and the in silico studies by molecular docking. Statistical Analysis Used: Microsoft Excel 2010 is used to calculate the mean and standard deviation for the ABTS and lipid peroxidation assay. Results: The antioxidant activity was found to be 20 µg/ml concentration in ABTS assay and 10 µg/ml concentration in lipid peroxidation assay. The in vitro anticancer activity was inspected using A549 cell lines by MTT assay. Tri cyclo [5,1,0,0 (2, 4) oct 5-ene 5 proponoic acid] 3,3,8,8 tetramethyl was selected as best lead against epidermal growth factor receptor of human based on the energy score calculated using the Auto-dock software. Conclusions: This study strongly supports that unexploited banana peels could be used to harvest promising lead molecules against non-small cell lung cancer.