Cell-free SLC30A10 messenger ribonucleic acid (mRNA) expression and their association with vitamin-D level among non-small cell lung cancer (NSCLC) patients-Reference-Cited by-同舟云学术

Cell-free SLC30A10 messenger ribonucleic acid (mRNA) expression and their association with vitamin-D level among non-small cell lung cancer (NSCLC) patients

Published:2023 Issue:Suppl 2 Volume:19 Page:S764-S769
ISSN:0973-1482
Container-title:Journal of Cancer Research and Therapeutics
language:en
Short-container-title:

Author:

Verma Amit K.¹,Naseeb Manal A.²,Basaqr Reem O.³,Albajri Eram A.²,Khan Mohammad Idreesh⁴,Dev Kapil¹,Beg Mirza Masroor Ali⁵⁶

Affiliation:

1. Department of Medical Biotechnology, Jamia Millia Islamia, New Delhi, India

2. Clinical Nutrition Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

3. Clinical Nutrition Department, College of Applied Medical Sciences –Jeddah, King Saud Bin Abdulaziz University for Health Sciences-Jeddah, Saudi Arabia (KSAU-HS), King Abdullah International Medical Research Center-Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia

4. Department of Clinical Nutrition, College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass 51921, Saudi Arabia

5. Centre for Promotion of Medical Research, Alatoo International University, Bishkek, Kyrgyzstan

6. Faculty of Medicine, Alatoo International University, Bishkek, Kyrgyzstan

Abstract

ABSTRACT Background: Lung cancer has been major cause of cancer related death and day by day Non-small cell lung cancer (NSCLC) cases are increasing globally. Present study explored the link between SLC30A10 mRNA expression with vitamin-D level among the NSCLC patients. Methods: Present study included newly diagnosed 100 NSCLC patients and 100 healthy controls. Quantitative real time PCR was performed to check the SLC30A10 mRNA expression after cDNA synthesis from extracted total RNA from serum sample. Vitamin-D level was also analyzed in all the NSCLC patients by electrochemiluminscence based immunoassay method. Results: Present research work observed decreased SLC30A10 mRNA expression (0.16 fold) among the NSCLC patients, decreased SLC30A10 mRNA expression was linked with advanced stage (0.15 fold, P < 00001) of disease and distant organ metastases (0.11 fold, P < 00001) compared to its contrast. Decreased level of vitamin-D was also observed with advanced stage (17.98 ng/ml, P < 00001) of disease and distant organ metastases (16.23 ng/ml, P < 00001) compared to its contrast. Positive correlation was observed between SLC30A10 mRNA expression with vitamin-D level among the NSCLC patients suggesting decrease or increase in SLC30A10 mRNA expression mau decreases or increase the vitamin-D level. NSCLC patients with vitamin-D deficiency had 0.14 reduced SCL30A10 mRNA expression while insufficient (P = 0 .06) and sufficient (P = 0.03) showed comparatively high SCL30A10 mRNA expression. Conclusion: Study concluded that down regulation of SLC30A10 mRNA and vitamin-D deficiency may involve in advancement of disease and distant organ metastases. It was also suggested that the decrease of increase in SLC30A10 expression may cause the decrease of increase in vitamin-D level among the NSCLC patients may be involved in disease severity and worseness of NSCLC disease.

Publisher

Medknow

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