Study of the biological activity of honey and royal jelly against Pseudomonas aeruginosa

Abstract

Abstract Background: The resistance of bacteria to antibiotics is an important problem that has attracted the attention of researchers. Also, the Pseudomonas aeruginosa is of great importance, especially in what it possesses of the possibility of inducing disease and overcoming the immune defenses. Objective: This study aimed to treat infection caused by P. aeruginosa with honey and royal jelly. Materials and Methods: Pseudomonas aeruginosa taken from the laboratories of the University of Baghdad diagnosed as a causative agent that is resistant to antibiotics. The diagnosis was carried out using some culture and microscopic, biochemical tests and the Vitek-2 test. After that, the sensitivity against some antibiotics by disk diffusion method was done, then the isolate was grown in different concentrations of local honey and royal jelly, to identify the effect of honey types on increasing the sensitivity toward the antibiotics. Results: The results showed that the isolate belonged to P. aeruginosa and was resistant to antibiotics The results showed that the presence of royal jelly, local honey, and their mixture at certain concentrations of 5%, 10%, and 15% increased the sensitivity toward most of the antibiotics disc zone (mm) and with a significant difference compared to the control, the sensitivity of the isolate grown in the culture medium containing 15% of royal jelly increased toward treatment SXI (Co-trimoxazole 25) is so that it becomes 27, and at the time the result of the isolate growing in the control medium was 13 and the same result (increasing the sensitivity) when using the antibiotic T (Tetracycline 30). Conclusion: Certain safe natural products, such as honey and royal jelly, exhibit antibacterial properties that have the potential to enhance the effectiveness of certain antibiotics when used in combination.