The Impact of Chitosan and Ozonated Water on the Remineralization of White Spot Lesion In Vitro

Abstract

Background: Dental caries is a complex, dynamical process that is controlled and initiated by a variety of variables. Objective: This study aimed to examine the ex-vivo remineralization impact of chitosan pretreated with ozonated water on artificially-induced incipient lesion. Materials and Methods: Artificial white spot caries was produced chemically by immersing 44 human teeth individually in demineralizing solution for 72 hours. Then the teeth were subjected to a 10-day pH cycle. Samples were assigned to four groups: (1) Control; (2) Chitosan (3) Ozonated water and (4) Chitosan application after treatment with 0.1 mg/ml ozonated water. Surface microhardness measurements were performed prior and after lesion formation then after treatment. Vickers microhardness tester and scanning electron microscope for representative teeth from the study sample were performed to assess enamel structure quantitatively and qualitatively. Results: The study’s findings showed that the sample from group 4 (chitosan after ozonated water treatment) had the highest level of enamel remineralization, and when compared to the control, there was a statistically significant difference(P<0.05). Conclusion: It could be stated that the combination of treatment of chitosan and ozone promoted the remineralization of artificially induced white spot lesion.