Estimation of myeloperoxidase levels in smokers, pan chewers, and normal individuals using enzyme-linked immunosorbent assay: An observational study

Abstract

Introduction: The effect of tobacco on human health is still a major concern worldwide. Myeloperoxidase (MPO) is an oxidative enzyme linked to inflammation and oxidative stress. There is limited information in the literature regarding the salivary and serum MPO levels in patients with betel quid chewing habit. The current study aimed to determine and compare MPO levels in the serum and saliva of smokers, betel quid chewers, and healthy controls. Materials and Methods: Twenty healthy controls, 20 individuals with smoking habits, and 20 individuals with betel quid chewing habits were included in the study. MPO levels in the collected serum and salivary samples were estimated using an ELISA kit (Immunology Consultants Laboratory Inc, Newberg, OR, USA). Statistical analysis was done using one-way analysis of variance followed by post hoc Tukey analysis and Kendall’s Tau correlation test. Results: In serum and saliva, the MPO levels of smokers and betel quid chewers were higher compared to healthy controls. A statistically significant correlation was observed between the serum and salivary MPO levels in healthy controls (P = 0.006). Conclusion: The current study demonstrated that salivary MPO is increased in individuals with smoking and betel quid chewing habits, which was previously unknown.