Effect of eyeliner and mascara use on tear film and meibomian glands

Abstract

PURPOSE: This study was conducted to see whether eyeliner, mascara, or combined eyeliner and mascara (EM) use affects tear production, tear film stability, and meibomian gland (MG) loss. METHODS: Two hundred and twenty healthy women underwent noninvasive tear break-up time (NTBUT) measurement, meibography, and Schirmer testing. Study groups were no makeup (NM) group, eyeliner-only group, mascara-only group, and those who used both EM. The one-way analysis of variance test was used for group comparisons. Chi-square test was used for meiboscale comparison. RESULTS: NTBUT (seconds) results were 11.5 ± 4.8 (no makeup), 21.3 ± 69 (eyeliner only), 21.8 ± 6.5 (mascara only), and 22.5 ± 7.0 (eyeliner–mascara). The differences between groups were significant (P < 0.0001). All makeup groups (eyeliner only, mascara only, eyeliner–mascara) had significantly diminished values compared with NM group (P < 0.001, P < 0.001 and P = 0.003, respectively). Schirmer test (millimeters) results were 22.7 ± 6.4 (NM group), 21.3 ± 6.9 (eyeliner only), 21.8 ± 6.5 (mascara only), and 22.5 ± 7.0 (eyeliner–mascara) with no significant differences between groups (P = 0.66). Meiboscale grading revealed that NM group had significantly lower values of MG loss compared with eyeliner-only (EO) (P = 0.01), mascara-only (MO) (P = 0.002), and eyeliner-mascara groups (P = 0.007). There were no significant differences between EO and MO (P = 0.31), EO and eyeliner–mascara (P = 0.39), or MO and eyeliner-mascara groups (P = 0.91). CONCLUSION: None of the makeup groups had changes in Schirmer wetting. All eye cosmetic groups have significant changes of NTBUT and meibography compared with NM subjects, and yet combined use of EM does not affect ocular surface more adversely than their separate use.