Estimation of the calcium content of intact tooth by decalcification and ethylene diamine tetra-acetic acid method – A analytical quantitative study

Abstract

Background: Teeth, one of the strongest structures of the human body, stands as reliable evidence in forensic odontology. The gender and age can be estimated from a tooth using various methods. We aimed to estimate the calcium content of an intact tooth and compare it with its average weight and dimension and also to find the possible correlation with the age and gender of an individual. Materials and Methods: A total of 180 extracted teeth were divided into group A (21-40), group B (41-60) and group C (61-80). Calcium estimation by decalcification followed by ethylene diamine tetra-acetic acid method analysis was performed. Results: Greatest calcium values were found in the males with mandibular premolars in group A, of maxillary premolars and mandibular molars in group B, as well as of maxillary canine, maxillary molars and mandibular incisors in group C. Greater calcium levels in the age of group B and C in mandibular incisors and male maxillary molars and in the jaws of group B, group C incisors and group C canines with P < 0.05. Calcium content and the weight of the teeth decreased with ageing. Conclusion: Calcium levels significantly varied in each tooth type, arch, gender and age.