Evaluation of in vitro anti-cancer potential and apoptotic profile of ethanolic plant extract of Wrightia tinctoria against oral cancer cell line

Abstract

Abstract Background: For decades, Wrightia tinctoria (W. tinctoria) has been important in the field of traditional medicine. The identification and isolation of numerous chemically potent and pharmaceutically advantageous phytochemicals isolated from this plant extract has contributed to its resurrection into evidence-based modern medicine. This plant is inexpensive and indigenous to the Indian subcontinent. Materials and Method: An in vitro study was carried out using KB (KERATIN-forming tumour cell line HeLa) oral cancer cell line. The control drug doxorubicin along with the test drug W. tinctoria was tested for anti-cancer potential by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and for apoptotic activity by using DNA fragmentation assay and western blotting assay. Results: The study was conducted using MTT assay to determine the cytotoxicity of plant extract against oral cancer cell line by determining the IC50 values at the end of 24 hours by the MTT calorimetric assay. The IC50 value of 48.89 and 9.62 was arrived for the W. tinctoria extract and doxorubicin, respectively, by using MTT calorimetric assay. DNA fragmentation assay showed a ladder pattern when treated with 59 μg/mL extract of W. tinctoria, and western blot analysis revealed the presence of cleaved caspase 3, confirming the apoptotic potential of the extract. Conclusion: The current study demonstrated that the plant extract has effective anti-cancer and apoptotic potential and can be used as a natural source for the production of new anti-cancer and cytotoxic agents against oral cancer.