Accuracy of Saliva as a Diagnostic Medium for Hepatitis B Virus Infection: A Quantitative ELISA Analysis

Author:

Onyia Nonso E.1,Okoh Mercy2,Omoregie Felix O.2,Ugiagbe Rose A.3,Ayinbuomwan Ekiye4

Affiliation:

1. Department of Oral Pathology, Medicine and Diagnosis, Faculty of Dentistry, College of Medical Sciences, University of Calabar, Calabar, Nigeria

2. Department of Oral and Maxillofacial Pathology and Medicine, University of Benin, Benin City, Edo State, Nigeria

3. Department of Internal Medicine, University of Benin, Benin City, Edo State, Nigeria

4. Department of Chemical Pathology, University of Benin, Benin City, Edo State, Nigeria

Abstract

Abstract Context: Diagnosis of hepatitis B virus infection (HBV) has been traditionally serum-based with its attendant risks and invasive procedure. Massive research interests are being directed to the use of saliva for screening, diagnosis, and monitoring of several infectious diseases, including HBV. Various reports suggest that saliva contains biomarkers that are comparable with that of sera. It can also be obtained with minimal risk to the clinician and patient as well. Aim: To determine the diagnostic accuracy of saliva in quantitative detection of hepatitis B surface antigen (HBsAg) using enzyme-linked immunosorbent assay (ELISA). Settings and Design: This cross-sectional analytical study was performed on HBV seropositive patients at the Gastroenterology clinic of the University of Benin Teaching Hospital, Benin City, Edo State. Materials and Methods: Under standardized conditions, equal amounts of blood and saliva samples of 43 HBsAg seropositive patients were analyzed using ELISA to quantitatively detect the concentration of HBsAg. Statistical Analysis Used: The analysis relied on Spearman’s correlation coefficient, linear regression analysis, and Bland–Altman plots to describe the correlational, predictive, and agreements between measurements of HBsAg in sera and saliva. The statistical significance was set at P < 0.05, while a 95% confidence level was used to construct intervals. Results: All participants had detectable levels of HBsAg in both saliva and serum with mean titers of 1.70 ± 0.35 ng/ml and 2.80 ± 0.77 ng/ml, respectively. Correlational and linear regression showed poor fit and predictive relationships of the HBsAg levels. Bland–Altman analysis showed good agreement and no significant bias in their diagnostic agreements. Conclusion: Saliva can be reliably used in screening and diagnosis of HBV infection. There was agreement in their levels independently and within their averages. It may be premature to rely on saliva for quantitative assay of HBsAg in treatment monitoring.

Publisher

Medknow

Reference39 articles.

1. Hepatitis B Virus DNA integration, chronic infections and hepatocellular carcinoma;Bousali;Microorganisms,2021

2. Evaluation of specificity and sensitivity of oral fluid for diagnosis of hepatitis B;Kh Khadse;J Clin Diagn Res,2016

3. Seroprevalence of hepatitis B infection in Nigeria:A national survey;Olayinka;Am J Trop Med Hyg,2016

4. Diagnostic accuracy of tests to detect hepatitis B surface antigen:A systematic review of the literature and meta-analysis;Amini;BMC Infect Dis,2017

5. Immunobiology and pathogenesis of hepatitis B virus infection;Iannacone;Nat Rev Reviews Immunol,2022

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3