An In vitro Assessment of Antibacterial Activity of Three Self-etching Primers Against Oral Microflora

Abstract

Aims This study aims to evaluate and compare the antibacterial activity of three self-etching primers (SEP), namely, Transbond plus, Reliance, and Gluma against commonly encountered oral microflora (Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus). Subjects and Methods The antibacterial activity of the three SEPs was examined against microorganisms using agar diffusion test (ADT) and minimum inhibitory concentration (MIC). In ADT, Whatman’s filter paper disc of 5 mm was loaded with primer and polymerized. This was placed on previously inoculated brain heart infusion and blood agar plates and was incubated for 48– 72 h at 37°C according to the microorganism. For assessing MIC serial dilution method was used. Statistical Analysis Used Data were analyzed with Kruskal–Wallis (P < 0.001) and Mann–Whitney tests. Results Only Transbond plus SEP and Reliance SEP produced a clear growth inhibition halo against S. mutans, L. acidophilus and A. viscosus. Gluma SEP did not show any growth inhibition halo against S. mutans, L. acidophilus, and A. viscosus. Conclusions TSEP and Reliance SEP did show antibacterial activity in an in vitro environment. Therefore, this study concludes that the use of these SEPs may contribute to a reduction in bacterial colonization.