Nitrofurantoin Sensitivity among Escherichia coli and Gram-Positive Cocci Isolated from Urinary Tract Infection

Abstract

BACKGROUND: Urine samples constitute a major proportion of the samples tested in routine diagnostic laboratories. Nitrofurination used to treat urinary tract infection (UTI) for both Gram-positive and negative bacteria. Resistance to UTI is growing with time, so continuous evaluation of antibiotic is urgent. MATERIALS AND METHODS: Mid-stream urine samples were collected from patients suspected with UTI. All these samples were inoculated on culture media aerobically at 37°C for overnight. A total of 100 bacteria of both Escherichia coli and Gram-positive cocci were diagnosed by general diagnostic bacteriological methods. Disc diffusion method used for antibiotic sensitivity test; two discs were tested, nitrofurination 300 μg and ceftazidime 30 μg. All the statistical analyses were done using SPSS 26 software and the Excel app. RESULTS: From the total of 100-positive specimens, the female was high prevalence with 72 specimens (72%), whereas males were just 28 specimens (28%) significantly (P < 0.0001). Nitrofurination was very sensitive in E. coli at 90.6% compared to G+ cocci at 33.3% (P < 0.0001). Ceftazidime showed no differences between E. coli 28.1% and G+ cocci 27.8% (P = 0.97). CONCLUSION: Nitrofurination is effective in both E. coli and G+ ve cocci, but it is more effective against Gram-negative bacteria (E. coli) 90.6% than G+ ve cocci 33.3% significantly (P < 0.0001). There is no significant correlation between ceftazidime and nitrofurantoin (r = −0.07, P = 0.44).