Quantitative analysis of remineralization of artificial carious lesions with commercially available toothpastes using EDAX: An in vitro study

Abstract

Introduction: Noninvasive approach for treating demineralized teeth is gaining importance in preventive dentistry. Various fluoridated and nonfluoridated remineralizing agents are utilized for treating early enamel carious lesions. Aim: To quantitatively assess the remineralizing potential of various dentifrices quantitatively from the array of products available in the market. Materials and Methods: Enamel blocks were prepared from seventy sound permanent teeth. The baseline amount of calcium, phosphorous, and fluoride was evaluated. After those samples were transferred in Modified TenCate’s solution for demineralization for 96 h, the amount of demineralization carried out to detect change in mineral content. Following this, samples were randomly grouped into five – Group 1: Fluoridated sensitive toothpaste, Group 2: Fluoridated toothpaste with Anticay Technology, Group 3: Nonfluoridated natural extracts toothpaste, Group 4: Nonfluoridated ayurvedic toothpaste, and Group 5: De-ionized water. Remineralizing agents applied twice daily for 28 days. The final mineral content of remineralized enamel was evaluated. Intra-group and inter-group comparisons were statistically analyzed using independent and paired sample t-test and analysis of variance. Results and Discussion: The mean calcium, phosphorous, fluoride, and Ca/P ratio of fluoridated and nonfluoridated toothpastes showed statistically significant difference (significant at P < 0.05). Intergroup comparison shows fluoridated toothpaste showing greater potential for remineralization (significant at P < 0.05). Conclusion: Fluoridated toothpaste with anticay technology showed better remineralizing capacity followed by sensitive and nonfluoridated natural toothpaste. Nonfluoridated ayurvedic toothpaste showed the least remineralizing potential.