A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using Direct RT-LAMP Assay-Reference-Cited by-同舟云学术

A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using Direct RT-LAMP Assay

Published:2023-11 Issue: Volume:12 Page:
ISSN:2277-9175
Container-title:Advanced Biomedical Research
language:en
Short-container-title:

Author:

Moazami Goodarzi Maryam¹,Taghizadeh Pirposhteh Razieh²,Ravan Hadi³,Vahidian Farnaz⁴,Kheirkhah Omolbani⁵,Fotouhi Ardakani Reza⁶,Fotouhi Fatemeh⁷

Affiliation:

1. Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, 3159915111 Karaj, Iran

2. Virology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran

3. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, 76169-14111 Kerman, Iran

4. Department of Biology, Science and Arts University, Yazd, Iran

5. Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran

6. Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, 3736175513, Iran

7. Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran

Publisher

Medknow

Reference26 articles.

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2. SARS-CoV-2 direct detection without RNA isolation with Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12;Garcia-Venzor;Front Med,2021

3. A loop-mediated isothermal amplification (LAMP) assay for the consensus detection of human pathogenic Campylobacter species;Babu;J Microbiol Methods,2020

4. Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction;Wei;Sci Rep,2021

5. Rapid isothermal amplification and portable detection system for SARS-CoV-2;Ganguli;Proc Natl Acad Sci U S A,2020