A Wound-Healing Effect of Nanoemulgel of Kangkung (Ipomoea Reptans, Poir) Leaf Extract in STZ Diabetic Rats

Abstract

ABSTRACT Context: The chronic diabetes mellitus (DM) condition may lead to diabetic wounds that increase morbidity in patients. Ipomoea reptans Poir leaves have been widely reported to possess anti-diabetic activity due to their flavonoid contents. To enhance drug penetration, a nanoemulgel preparation was formulated. Aims: This study aimed to evaluate the activity of nanoemulgel preparations of Ipomoea reptans Poir leaf extract on diabetic and non-diabetic wound-healing using male Wistar rats. Settings and Design: This research was an experimental study with a post-test only control group design. Materials and Methods: The rats (n = 32) were randomly divided into two groups: diabetic (induced by 40 mg/kg BW STZ) and non-diabetic model. Each model consisted of four groups: normal, positive control, I. reptans leaf extract (IRLE), and nanoemulgel of I. reptans leaf extract (NIRLE). All the animals studied were shaved from the back, and a 2.5 × 0.5 cm full-thickness excision wound was made. IRLE and NIRLE were administered daily and observed for the wound-healing process. Statistical Analysis Used: The one-way analysis of variance with the Tukey post-hoc test was used for the statistical analysis. Results: A NIRLE formulation has been developed to produce a preparation that meets the physical requirements. IRLE and NIRLE possessed wound-healing activity in normal and diabetic rat models. However, the wound-healing process in diabetic rats treated with NIRLE was faster than those with IRLE. Conclusions: NIRLE increased the activity of wound-healing effect of I. reptans leaves on diabetic rats in comparison with the extract form.