Comparing Oral Microbiota Changes after Pulpotomy/Pulpectomy in Pediatric Patients

Abstract

ABSTRACT Background: Pediatric dental procedures like pulpotomy and pulpectomy are commonly performed to treat pulp-related dental issues in primary teeth. The choice of instrumentation, such as traditional rotary instruments or laser technology, can impact the oral microbiota. This study aims to compare the changes in oral microbiota following pulpotomy/pulpectomy in pediatric patients treated with traditional rotary instruments versus laser technology. Materials and Methods: A total of 60 pediatric patients, aged 4–10 years, requiring pulpotomy or pulpectomy were randomly assigned to two groups: group A (traditional) and group B (laser). In group A, traditional rotary instruments were used for pulp removal, while in group B, a dental laser was utilized. Saliva and plaque samples were collected before the procedure (baseline), immediately after the procedure, and at the one-month follow-up. Microbial deoxyribonucleic acid (DNA) was extracted and analyzed using 16S ribosomal ribonucleic acid (rRNA) sequencing to assess changes in the oral microbiota. Results: At baseline, both groups exhibited similar microbial diversity and composition. However, immediate post-procedure results showed a significant decrease in microbial diversity in both groups, with group B (laser) demonstrating a more pronounced reduction. At the one-month follow-up, group A (traditional) showed a partial recovery of microbial diversity, while group B (laser) continued to exhibit lower microbial diversity compared to baseline. Notably, group B (laser) displayed a shift in microbial composition with a decrease in pathogenic species and an increase in beneficial bacteria compared to group A. Conclusion: This study highlights that both traditional rotary instruments and laser technology have a significant impact on the oral microbiota of pediatric patients following pulpotomy/pulpectomy. While both methods initially reduce microbial diversity, laser treatment appears to have a more sustained effect, favoring the growth of beneficial bacteria over time. Further research is needed to assess the long-term clinical implications of these microbiota changes for the oral health of pediatric patients.