Evaluation of the diagnostic performance of circulating microRNAs for the diagnosis of autism spectrum disorders

Author:

Abdelkarem Omneya A.I.1,Zaki Moyassar A.1,Elwafa Reham A.H.A.2,Elmaksoud Marwa A.3,El Banna Alaa1

Affiliation:

1. Chemical Pathology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt

2. Clinical and Chemical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt

3. Neurology Unit, Department of Pediatrics, Faculty of Medicine, Alexandria University, Alexandria, Egypt

Abstract

Background Autism spectrum disorder (ASD) is a neurodevelopmental disorder with genetic and environmental influences. Recently, microRNA (miRNA), has been identified as a potential contributor to the pathogenesis of several neurodevelopmental abnormalities, including ASD. Aim This study aimed to assess the diagnostic performance of a miRNA panel (miR-146a-5p, miR-106b-5p, miR-148a-5p) in ASD diagnosis. Patients and methods Fifty children, 16 with ASD and 34 normally developing were enrolled in the study. Relative expression levels of plasma miR-146a-5p, miR-106b-5p, and miR-148a were determined by real-time reverse transcription-quantitative PCR. Receiver operator characteristic analysis was done to evaluate the diagnostic performance of the studied panel. Functional enrichment analysis was conducted to detect the relationship between miRNA targets and relevant pathways. Results Results showed significantly higher levels of all three miRNAs in ASD children compared to controls. Receiver operator characteristic analysis indicated high diagnostic accuracy for miR-106b-5p area under a curve (AUC)=0.959], miR-146a-5p (AUC=0.980), and miR-148a-5p (AUC=0.995). Functional analysis revealed enrichment of miRNA targets in pathways related to neurodevelopment, such as FoxO, PI3K-Akt, and HIF-1 signaling. MiR-146a-5p targets were enriched in innate immunity pathways like Toll-like receptor and chemokine signaling. These findings suggest a complex interplay between neuroepigenetics and neurogenetic pathways in ASD pathogenesis. Conclusion The proposed miRNA panel shows promise for distinguishing children with ASD from normally developing children. Moreover, the study highlights the intricate relationship between miRNAs and ASD-related pathways, emphasizing the need for further large-scale validation studies. Integrating genetic, epigenetic, and environmental factors may improve our understanding and management of ASD.

Publisher

Medknow

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3