In vitro Evaluation of Remineralization Efficacy of Fluoridated and Nonfluoridated Toothpastes on Demineralized Enamel Surface

Abstract

Aim: The aim of the present study was to compare the remineralization efficacy of fluoridated and nonfluoridated toothpaste on artificially demineralized enamel. Materials and Methods: Buccal surface of eighty extracted human premolars was treated with demineralizing solution for 96 h and they were randomly distributed into four groups. Group 1 was control, Group 2 was brushed with Xyliwhite toothpaste (fluoride free), Group 3 was brushed with Superbee Propolis toothpaste (fluoride free), and Group 4 was brushed with Amflor Pro toothpaste (Fluoridated). Brushing was performed for 2 min twice daily with a powered toothbrush for 7 days. After every brushing, the specimens were cleaned and stored in artificial saliva at room temperature. The enamel surface microhardness was measured with Vickers hardness test postdemineralization and posttreatment and a comparative analysis was made. Results: One-way analysis of variance F-test showed a statistically significant difference in remineralization efficacy among the groups (P < 0.001). Tukey’s post hoc test showed that Group 2, Group 3, and Group 4 have higher remineralization efficacy as compared to control (P < 0.001). Maximum remineralization efficacy was seen with Group 4. Conclusion: Fluoridated and nonfluoridated toothpastes have the potential to remineralize the demineralized enamel surface.