Effect of orlistat versus lipo-6 black on the testis of adult obese albino rats: histological and histomorphometric study

Abstract

Background Obesity is a health problem with increasing incidence. Excess fat is the most common factor that contributes to its pathogenesis. Aim To evaluate the effect of obesity on the testis of male rats and compare the possible ameliorative effect of orlistat versus lipo-6. Materials and methods Fifty-six adult male rats were used. The control group ‘eight rats’ received a standard diet (SD) with no treatment for 90 days. The experimental group ‘48 rats’ received high-fat diet (HFD) for 60 days for obesity induction. Obese rats were subdivided into three groups (each group a and b). IIa received SD and IIb received HFD. IIIa received SD+orlistat and IIIb received HFD+orlistat. IVa received SD+lipo-6. IVb received HFD+lipo 6. The drugs were used for 30 days. Testicular specimens were subjected to histological, histomorphometrical, and immunohistochemical examinations. Data on obesity index, testis weight, abdominal circumference sperm percentage, and histomorphometry were analyzed statistically. Results Subgroups IIIa and IVa showed restoration of the structural integrity of the seminiferous tubules with the normal distribution of collagen fibers. Sections of subgroup IIIa showed negative caspase-3 immunostaining near the control group. Lee obesity index and percentage of abnormal sperm were close to control. Conclusion Orlistat and lipo-6 with diet adjustment reduced the severity of testicular damage induced by obesity.