Comparing and Evaluating the Antimicrobial Efficacy of Endodontic Root Canal Sealers against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans at 24, 48, and 72 h: An in vitro study

Abstract

Introduction: Examining the effectiveness of root canal sealants next to Staphylococcus aureus, Enterococcus faecalis, and Candida albicans using the agar well diffusion technique was the focus of this research. Materials and Methods: Antimicrobial effectiveness was evaluated using a selection of endodontic root canal sealers, including AH Plus, Endomethasone, Sealapex, and GuttaFlow. Antimicrobial zones of reserve were evaluated at 24, 48, and 72 h using the agar well diffusion method. The effectiveness of sealers against a few common germs was evaluated using the ANOVA and Tukey’s post hoc testing. Results: Large antimicrobial inhibitory zones were seen across all experimental groups. Endomethasone sealer showed the largest inhibition zones, measuring 16.40 (±1.02) mm at 24 h, 23.33 (±1.66) mm at 48 h, and 28.93 (±1.97) mm at 72 h. Sealapex and AH Plus came in the second and third, respectively; GuttaFlow showed no inhibition zones. Conclusions: The antibacterial efficacy of Endomethasone sealer against S. aureus, E. faecalis, and C. albicans was the greatest at 24, 48, and 72 h.