Blood-derived products can moderate the activity of neutrophils isolated after biomaterial implantation in a sheep model

Author:

NGOC DOMINIKA NGUYEN,DRZEWIECKA BEATA,JUNKUSZEW ANDRZEJ,PATKOWSKI KRZYSZTOF,SZPONDER TOMASZ,SZYMCZAK BARTŁOMIEJ,NOWAKIEWICZ ANETA,PODWYSOCKA SANDRA,WESSELY-SZPONDER JOANNA

Abstract

Neutrophils play a pivotal role in both the inflammatory phase and subsequent repair processes, making their activity crucial after contact with biomaterials. Excessive neutrophil activation can lead to complications, including implant rejection, highlighting the importance of modulating their activity. This study investigates the long-term interaction of circulating ovine neutrophils with titanium (Ti) and silicon-doped layer (Si-DLC) coated implants. Additionally, the effects of platelet-rich plasma (PRP), platelet-poor plasma (PPP), and antimicrobial neutrophil crude extract (ANE) on neutrophil activity were assessed. Twelve female sheep were divided into Ti, Si-DLC implant and a control group. Neutrophils were stimulated with PRP, PPP, ovine ANE (oANE), or rabbit ANE (rANE), and their enzymatic activity and reactive oxygen and nitrogen species (RONS) generation were evaluated. Hematological parameters were also analyzed. Results showed no adverse effects at implant sites, and all hematological parameters remained within reference values. Ti and Si-DLC implants did not alter neutrophil activity. PRP significantly increased neutrophil activity and RONS production, while PPP decreased activity. oANE reduced enzymatic activity and RONS generation, indicating potential antiinflammatory effects. rANE showed varied effects on neutrophil function. Both ANEs lacked antimicrobial activity against tested pathogens. This study demonstrates the hemocompatibility of Ti and Si-DLC implants and highlights the potential of blood-derived products in modulating the inflammatory response to implants.

Publisher

Medycyna Weterynaryjna - Redakcja

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3