Development of a Competitive Lateral Flow Immunoassay for Progesterone in Dairy Cows’ Milk

Abstract

The routine monitoring of progesterone, a hormone that plays important roles during pregnancy, has become increasingly important in dairy cows. In this study, 11α-OH-progesterone-hemisuccinate-BSA was used to immunize Balb/c mice for the preparation of mouse anti-progesterone monoclonal antibodies (mAbs). A competitive lateral flow immunoassay for progesterone was developed with the use of purified mAbs. Standard progesterone solutions or milk samples containing different concentrations of progesterone were added to the sample pad of the test strip. A bright or weak test line represented progesterone levels <3 ng> </tr> <tr> <td class="keywords">Key words: progesterone; lateral flow immunoassay; pregnancy diagnosis; dairy cows</td> </tr> </tbody>