Application of real-time PCR for DNA identification of ruminants in raw animal products

Abstract

According with current regulations it is possible export of processed animal proteins produced from tissues other animals than ruminants. In order to ensure appropriate control studies were undertaken to adopt of method real-time PCR used for analysis of processed products of animal origin. The material consisted of raw animal by-products of beef, pork and poultry. In the assays mitochondrial DNA was used. Modified method allows to detect the DNA of ruminants at the level of 0.025%. Based on obtained results of the validation studies were found that the real-time PCR method can be used in routine tests for identification of ruminant DNA in raw animal by-products..