Comparison of the Vcheck and IMMULITE 2000 methods for cortisol measurement in canine serum

Abstract

The aim of this study was to compare canine cortisol results obtained by the Vcheck method with those obtained by the IMMULITE 2000 immunoassay, which had previously been validated for canine serum. The concentration of cortisol in 44 canine serum samples was determined concurrently by the Vcheck and IMMULITE methods, the latter as the reference method. Cortisol values were compared using Pearson’s correlation analysis and simple regression analysis. Agreement between the two methods was calculated with a Bland-Altman plot. According to regression analysis and the Bland-Altman plot, the two methods gave comparable results. The results of the Vcheck method were comparable to those of the IMMULITE 2000 reference method, so the Vcheck may be used as an alternative assay to evaluate serum cortisol concentration in dogs for the diagnosis of adrenal disease.