Rapid assembly of multiple DNA fragments through direct transformation of PCR products into E. coli and Lactobacillus-Reference-Cited by-同舟云学术

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Rapid assembly of multiple DNA fragments through direct transformation of PCR products into E. coli and Lactobacillus

Published:2014-11 Issue: Volume:76 Page:40-46
ISSN:0147-619X
Container-title:Plasmid
language:en
Short-container-title:Plasmid

Author:

Cao Pinghua,Wang Lei,Zhou Guangxian,Wang Yaoyue,Chen Yulin

Funder

National Natural Science Foundation of China

China Agriculture Research System

National Special Research Fund for Non-profit Sector (Agriculture) of China

Publisher

Elsevier BV

Subject

Molecular Biology

Reference18 articles.

1. Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids;Bryksin;Biotechniques,2010

2. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis;Canosi;Mol. Gen. Genet,1978

3. One-step random mutagenesis by error-prone rolling circle amplification;Fujii;Nucleic Acids Res,2004

4. USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products;Geu-Flores;Nucleic Acids Res,2007

5. Enzymatic assembly of DNA molecules up to several hundred kilobases;Gibson;Nat. Methods,2009

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2. Cloning, expression and purification of the low-complexity region of RanBP9 protein;Protein Expression and Purification;2020-08

3. High‐efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme;Microbial Biotechnology;2020-06-29

4. Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension;BioTechniques;2020-06

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