An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology

Author:

Skowron Piotr M.,Krawczun Natalia,Żebrowska Joanna,Krefft Daria,Żołnierkiewicz Olga,Bielawa Marta,Jeżewska-Frąckowiak Joanna,Janus Łukasz,Witkowska MałgorzataORCID,Palczewska Małgorzata,Zylicz-Stachula AgnieszkaORCID

Funder

National Centre for Research and Development – Poland

Publisher

Elsevier BV

Subject

Medical Laboratory Technology,Clinical Biochemistry

Reference7 articles.

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid;Chang;J. Bacteriol.,1978

2. A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications;Skowron;Mater. Sci. Eng. C.,2020

3. Data regarding a new, vector-enzymatic DNA fragment amplification-expression technology for the construction of artificial, concatemeric DNA, RNA and proteins, as well as biological effects of selected polypeptides obtained using this method;Skowron;Data Brief,2020

4. P. Skowron, A. Żylicz-Stachula, O. Żołnierkiewicz, M. Skowron, Ł. Janus, J. Jeżewska-Frąckowiak, A. SzymańskaSposób uzyskiwania białka poliepitopowego oraz wektor DNA do realizacji tego sposobu (A method of obtaining a polyepitopic protein as well as a DNA vector for embodying this method), Republic of Poland patent office no 228341 (2018).

5. Skowron, A.Z.-S., O. Zolnierkiewicz, M. Skowron, L. Janus, J. Jezewska-Frackowiak, D. Krefft, D. Nidzworski, K. Szemiako, N. Maciejewska, M. Nowak, A. SzymanskaA method of obtaining a polyepitopic protein as well as a DNA vector for embodying this method, PCT patent application WO 2015162560 A1(2015).

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