Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates

Author:

Stasevich Timothy J.,Mueller Florian,Michelman-Ribeiro Ariel,Rosales Tilman,Knutson Jay R.,McNally James G.

Publisher

Elsevier BV

Subject

Biophysics

Reference43 articles.

1. Fluorescence recovery after photobleaching: application to nuclear proteins;Houtsmuller;Adv. Biochem. Eng. Biotechnol.,2005

2. FRAP analysis of binding: proper and fitting;Sprague;Trends Cell Biol.,2005

3. Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins;Beaudouin;Biophys. J.,2006

4. FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?;Mueller;Curr. Opin. Cell Biol.,2010

5. Conditions for using FRAP as a quantitative technique—influence of the bleaching protocol;Trembecka;Cytometry A.,2010

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