Protocol to measure protein-RNA binding using double filter-binding assays followed by phosphorimaging or high-throughput sequencing
Author:
Funder
National Institutes of Health
Publisher
Elsevier BV
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference24 articles.
1. Principles and pitfalls of high-throughput analysis of microRNA-binding thermodynamics and kinetics by RNA Bind-n-Seq;Jouravleva;Cell Rep. Methods,2022
2. Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions;Hellman;Nat. Protoc.,2007
3. Sensing the heat: the application of isothermal titration calorimetry to thermodynamic studies of biomolecular interactions;Ladbury;Chem. Biol.,1996
4. Fluorescence polarization anisotropy to measure RNA dynamics;Shi;Methods Enzymol.,2009
5. A double-filter method for nitrocellulose-filter binding: application to protein-nucleic acid interactions;Wong;Proc. Natl. Acad. Sci. USA,1993
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