Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells
Author:
Publisher
Elsevier BV
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference6 articles.
1. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing;Labun;Nucleic Acids Res.,2019
2. Inference of CRISPR edits from Sanger trace data;Conant;CRISPR J,2022
3. Easy quantitative assessment of genome editing by sequence trace decomposition;Brinkman;Nucleic Acids Res.,2014
4. Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX;Yu;Biotechnol. Lett.,2016
5. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection;Liang;J. Biotechnol.,2015
Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Optimized CRISPR-based knockout in BeWo cells;Placenta;2024-07
2. CRISPR/Cas9-mediated knock out of ITGB6 in human OSCC cells reduced migration and proliferation ability;Head & Face Medicine;2024-06-18
3. CHD4 acts as a prognostic factor and drives radioresistance in HPV negative HNSCC;Scientific Reports;2024-04-09
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