Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi-Reference-Cited by-同舟云学术

Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

Published:2022-06 Issue:2 Volume:3 Page:101324
ISSN:2666-1667
Container-title:STAR Protocols
language:en
Short-container-title:STAR Protocols

Author:

Picchi-Constante Gisele Fernanda Assine,Hiraiwa Priscila Mazzocchi,Marek Martin,Rogerio Vanessa Zulkievicz,Guerra-Slompo Eloise Pavão,Romier Christophe,Zanchin Nilson Ivo Tonin^ORCID

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference12 articles.

1. Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants;Burle-Caldas;Int. J. Parasitol.,2018

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3. Biological aspects of the Dm 28c clone of Trypanosoma cruzi after metacyclogenesis in chemically defined media;Contreras;Memórias Do Inst. Oswaldo Cruz,1988

4. Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi;Darocha;Mol. Biochem. Parasitol.,2004

5. CRISPR/Cas9-Induced disruption of paraflagellar rod protein 1 and 2 genes in Trypanosoma cruzi reveals their role in flagellar attachment;Lander;MBio,2015

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