Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay-Reference-Cited by-同舟云学术

Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay

Published:2022-12 Issue:4 Volume:3 Page:101771
ISSN:2666-1667
Container-title:STAR Protocols
language:en
Short-container-title:STAR Protocols

Author:

Mutavhatsindi Hygon,Riou Catherine^ORCID

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference13 articles.

1. An optimized flow cytometry protocol for simultaneous detection of T cell activation induced markers and intracellular cytokines: application to SARS-Cov-2 vaccinated individuals;Altosole;medRxiv,2022

2. Immuno-monitoring of CD8+ T cells in whole blood versus PBMC samples;Appay;J. Immunol. Methods,2006

3. Association of HIV-specific and total CD8+ T memory phenotypes in subtype C HIV-1 infection with viral set point;Burgers;J. Immunol.,2009

4. Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies;Hanekom;J. Immunol. Methods,2004

5. A sensitive whole blood assay detects antigen-stimulated cytokine release from CD4+ T cells and facilitates immunomonitoring in a phase 2 clinical trial of Nexvax2 in coeliac disease;Hardy;Front. Immunol.,2021

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