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An optimized protocol for the retroviral transduction of mouse CD4 T cells

  • Published:2021-09 Issue:3 Volume:2 Page:100719
  • ISSN:2666-1667
  • Container-title:STAR Protocols
  • language:en
  • Short-container-title:STAR Protocols

Author:

Eremenko Ekaterina,Taylor Zoe V.,Khand Bishnu,Zaccai Shir,Porgador Angel,Monsonego Alon

Funder

National Agri-Food Biotechnology Institute

Ministry of Science and Technology, Israel

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference14 articles.

1. IL15 enhances CAR-T cell antitumor activity by reducing mTORC1 activity and preserving their stem cell memory phenotype;Alizadeh;Cancer Immunol. Res.,2019

2. A short CD3/CD28 costimulation combined with IL-21 enhance the generation of human memory stem T cells for adoptive immunotherapy;Alvarez-Fernández;J. Transl. Med.,2016

3. Retroviruses;Coffin,1997

4. Overview of the retrovirus transduction system;Cepko;Curr. Protoc. Mol. Biol.,2001

5. Retroviral vectors for high-level transgene expression in T lymphocytes;Engels;Hum. Gene Ther.,2003
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