Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures

Author:

Piffer Alicia C.,Reis Flavia C.G.,Moyrand Frédérique,Montalvão Bruna,Rodrigues Marcio L.,Janbon Guilhem,Rizzo JulianaORCID

Funder

Institut Pasteur

FAPERJ

Agence Nationale de la Recherche

Publisher

Elsevier BV

Reference11 articles.

1. Cryptococcus extracellular vesicles properties and their use as vaccine platforms;Rizzo;J. Extracell. Vesicles,2021

2. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans;Rizzo;mBio,2023

3. A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in Cryptococcus gattii;Reis;mSphere,2019

4. Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells;Reis;Microbiol. Spectr.,2021

5. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches;Welsh;J. Extracell. Vesicles,2024

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