Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation technology

Author:

Li Na,Yan Yumeng,Wu Bowen,Wang Jifeng,Yang FuquanORCID

Funder

Ministry of Science and Technology of the People's Republic of China

National Key Research and Development Program of China

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference15 articles.

1. Separation and characterization of extracellular vesicles from human plasma by asymmetrical flow field-flow fractionation;Wu;Anal. Chim. Acta,2020

2. Differential proteomic patterns of plasma extracellular vesicles show potential to discriminate beta-thalassemia subtypes;Li;iScience,2023

3. Quantitative Proteomics Using Isobaric Labeling: A Practical Guide;Chen;Dev. Reprod. Biol.,2021

4. TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples;Li;Nat. Methods,2020

5. 29-Plex tandem mass tag mass spectrometry enabling accurate quantification by interference correction;Sun;Proteomics,2022

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