Protocol for altering connexin hemichannel function in primary chicken lens fiber cells using high-titer retroviral RCAS(A) infection

Author:

Du Yu,Acosta Francisca M.,Zhang Jianping,Tong Yuxin,Quan Yumeng,Gu Sumin,Jiang Jean X.ORCID

Funder

National Institutes of Health

Welch Foundation

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference10 articles.

1. Mechanosensitive collaboration between integrins and connexins allows nutrient and antioxidant transport into the lens;Liu;J. Cell Biol.,2020

2. Mitogen-activated Protein Kinase (MAPK) Activated by Prostaglandin E2 Phosphorylates Connexin 43 and Closes Osteocytic Hemichannels in Response to Continuous Flow Shear Stress;Riquelme;J. Biol. Chem.,2015

3. Connexin hemichannels mediate glutathione transport and protect lens fiber cells from oxidative stress;Shi;J. Cell Sci.,2018

4. Use of retroviruses to express connexins;Jiang;Methods Mol. Biol.,2001

5. Correlation of cell strain in single osteocytes with intracellular calcium, but not intracellular nitric oxide, in response to fluid flow;Rath;J. Biomech.,2010

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