Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification

Author:

Han XingfaORCID,Burger Laura L.ORCID,Garcia-Galiano DavidORCID,Moenter Suzanne M.ORCID,Myers Martin G.ORCID,Olson David P.,Elias Carol F.ORCID

Funder

National Institutes of Health

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference21 articles.

1. Defining the transcriptional targets of leptin reveals a role for Atf3 in leptin action;Allison;Diabetes,2018

2. TRAP-seq defines markers for novel populations of hypothalamic and brainstem LepRb neurons;Allison;Mol. Metab.,2015

3. TRAP-rc, translating ribosome affinity purification from rare cell populations of Drosophila embryos;Bertin;J. Vis. Exp.,2015

4. Identification of genes enriched in GnRH neurons by translating ribosome affinity purification and RNAseq in mice;Burger;Endocrinology,2018

5. Prenatal androgen exposure alters the developmental trajectory of both GnRH neuron and preoptic area RNA transcripts in female mice;Burger,2020

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