Effect of C-terminal His-tag and purification routine on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of the bacteriophage T5-Reference-Cited by-同舟云学术

Effect of C-terminal His-tag and purification routine on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of the bacteriophage T5

Published:2019-03 Issue: Volume:124 Page:810-818
ISSN:0141-8130
Container-title:International Journal of Biological Macromolecules
language:en
Short-container-title:International Journal of Biological Macromolecules

Author:

Kutyshenko Victor P.,Mikoulinskaia Galina V.^ORCID,Chernyshov Sergei V.,Yegorov Alexander Y.,Prokhorov Dmitry A.,Uversky Vladimir N.

Funder

RFBR

Publisher

Elsevier BV

Subject

Molecular Biology,General Medicine,Biochemistry,Structural Biology

Reference38 articles.

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2. Immobilized-metal affinity chromatography (IMAC): a review;Block;Methods Enzymol.,2009

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4. Immobilized metal ion affinity chromatography: a review on its applications;Cheung;Appl. Microbiol. Biotechnol.,2012

5. Purification of His-tagged proteins;Spriestersbach;Methods Enzymol.,2015

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