High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli-Reference-Cited by-同舟云学术

High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli

Published:2009-02 Issue:2 Volume:63 Page:120-127
ISSN:1046-5928
Container-title:Protein Expression and Purification
language:en
Short-container-title:Protein Expression and Purification

Author:

Brandis John W.,Johnson Kenneth A.

Publisher

Elsevier BV

Subject

Biotechnology

Reference19 articles.

1. Rapid quench kinetic analysis of polymerases, adenosinetriphosphatases, and enzyme intermediates;Johnson;Methods Enzymol.,1995

2. Recombinant protein expression in Escherichia coli;Baneyx;Curr. Opin. Biotechnol.,1999

3. Strategies for achieving high-level expression of genes in Escherichia coli;Makrides;Microbiol. Rev.,1996

4. Improved M13 phage and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors;Yanish-Perron;Gene,1985

5. Cloning of the gene for thermostable Thermus aquaticus YT-1 DNA polymerase and its expression in Escherichia coli;Patrushev;Mol. Biol. (Mosk),1993

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli;STAR Protocols;2021-03

2. Remdesivir Is Effective in Combating COVID-19 because It Is a Better Substrate than ATP for the Viral RNA-Dependent RNA Polymerase;iScience;2020-12

3. Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication;Journal of Biological Chemistry;2020-12

4. Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs;Journal of Biological Chemistry;2020-07

5. The human mitochondrial single-stranded DNA-binding protein displays distinct kinetics and thermodynamics of DNA binding and exchange;Journal of Biological Chemistry;2017-08