Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

Author:

Esposito Dominic,Mehalko Jennifer,Drew Matthew,Snead Kelly,Wall Vanessa,Taylor Troy,Frank Peter,Denson John-Paul,Hong Min,Gulten Gulcin,Sadtler Kaitlyn,Messing Simon,Gillette William

Funder

National Cancer Institute, National Institutes of Health

Publisher

Elsevier BV

Subject

Biotechnology

Reference11 articles.

1. Serology assays to manage COVID-19;Krammer;Science,2020

2. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation;Wrapp;Science,2020

3. SARS-CoV-2 seroconversion in humans: a detailed protocol for a serological assay, antigen production, and test setup;Stadlbauer;Curr Protoc Microbiol,2020

4. Standardization of enzyme-linked immunosorbent assays for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling;Klumpp-Thomas;medRxiv:2020.2005.2021.20109280,2020

5. Comparison of the Coomassie brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-glycosylated and glycosylated proteins;Fountoulakis;J. Biochem. Biophys. Methods,1992

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