Development of a rapid, single-step procedure using protein G affinity chromatography to deplete fetal calf serum of its IgG and to isolate murine IgG1 monoclonal antibodies from supernatants of hybridoma cells

Author:

Aybay Cemalettin,Imir Turgut

Publisher

Elsevier BV

Subject

Immunology,Immunology and Allergy

Reference10 articles.

1. Baumgarten, H., 1992. Protein A/Protein G column chromatography. In: Peters, J.H., Baumgarten, J.H. (Eds.), Monoclonal Antibodies. Springer-Verlag, New York, p. 264.

2. Cross flow diafiltration of serum with basal medium suitable for growth of hybridomas, sterilization and protein reduction;Jungbauer;Dev. Biol. Stand.,1986

3. Effect of serum concentration on hybridoma cell growth and monoclonal antibody production at various initial cell density;Lee;Hybridoma,1989

4. Serum can act as a shear protecting agent in agitated hybridoma cell cultures;Lee;Hybridoma,1989

5. Loss of antibody productivity during long-term cultivation of a hybridoma cell line in low serum and serum-free media;Ozturk;Hybridoma,1990

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